from tkinter import *
import tkinter as tk

创建计数器

def a():    

    def counter_label(label):            
        counter=0
        def count():

            global counter
            counter += 1
            label.config(text=str(counter))
            label.after(1000,count)
        count()

    label=tk.Label(frame,fg="red")
    label.grid(row=0,column=1)

def create_widgets_in_frame():

为框架创建小部件

    Label(frame,text="TIME:",padx=25,pady=50,font=("arial",20,"bold"),bg="red",width=10).grid(row=0,column=0,sticky=W)    
    Button(frame,text="START",padx=25,pady=50,font=("arial",20,"bold"),command=a,bg="red",width=10).grid(row=1,column=0,sticky=E)
    Button(frame,text="STOP",padx=25,pady=50,font=("arial",20,"bold"),bg="red",width=10).grid(row=1,column=1,sticky=E)
    Button(frame,text="EXIT",padx=25,pady=50,font=("arial",20,"bold"),command = quit_program,bg="red",width=10).grid(row=1,column=2,sticky=E)    

def call_frame_on_top():
    frame.grid(column=0, row=0, padx=20, pady=5, sticky=W)

def quit_program():
    root_window.destroy()

root_window=Tk()
root_window.geometry("1500x1000+0+0")
window_width = 1500
window_heigth = 1000

在根窗口内创建框架以容纳其他 GUI 元素。所有框架必须在主程序中创建，否则无法在函数中访问它们。

frame=Frame(root_window,bg="blue", width=window_width, height=window_heigth)
frame.grid(column=0, row=0, padx=20, pady=5, sticky=W)

创建框架小部件

create_widgets_in_frame()

root_window.configure(background="blue")
root_window.mainloop()

功能中a()你实际上从未调用过counter_label，所以它不会开始计数。你需要定义counter函数外部的变量，以便您可以使用global关键词。

这是您修改后的代码：

from tkinter import *
import tkinter as tk

counter = 0                             #Defining counter so you can use it with global
def a():    
    def counter_label(label):            
        counter=0
        def count():
            global counter
            counter += 1
            label.config(text=str(counter))
            label.after(1000,count)
        count()

    label=tk.Label(frame,fg="red")
    label.grid(row=0,column=1)
    counter_label(label)                #Calling the counter_label function

...